Cardioprotective Effects of Cichorium intybus and Nyctanthes arbor-tristis Against Doxorubicin-Induced Cardiotoxicity in Experimental Animal Models (Code-T0289)

Introduction: Cardiovascular diseases, including myocardial infarction, remain a leading cause of mortality worldwide. The cardiotoxicity induced by doxorubicin, a widely used chemotherapeutic agent, necessitates the search for natural cardioprotective agents. Cichorium intybus and Nyctanthes arbor-tristis, known for their antioxidant and anti-inflammatory properties, have shown potential in mitigating cardiac damage.

Aims and Objectives

1. To evaluate the cardioprotective effect of Cichorium intybus against doxorubicin induced cardiac injury
2. To evaluate the cardioprotective effect of Nyctanthes arbor-tristis against doxorubicin induced cardiac injury

Methods
Adult male Wistar rats were divided into six groups, including normal control, doxorubicin-treated, and test groups receiving different doses of plant extracts alongside doxorubicin. Cardiotoxicity was assessed through biochemical markers, including AST, ALT, LDH, cardiac troponins, inflammatory cytokines (TNF-α, IL-6), oxidative stress markers (MDA, GSH, NO, TAC), lipid profile, and histopathological examination of myocardial tissue. Statistical analysis was performed using ANOVA with p < 0.005 considered significant.

Results
Doxorubicin-induced cardiotoxicity was evidenced by elevated oxidative stress, inflammatory markers, and myocardial enzyme levels. Treatment with Cichorium intybus and Nyctanthes arbor-tristis significantly improved biochemical parameters, reduced lipid peroxidation, and restored antioxidant enzyme activity. Histopathological analysis demonstrated reduced myocardial damage in treated groups.

Conclusion
Cichorium intybus and Nyctanthes arbor-tristis exhibit significant cardioprotective effects against doxorubicin-induced cardiotoxicity, likely through antioxidant and anti-inflammatory mechanisms. These findings support their potential use as adjuncts in cardioprotection.

Keywords
Cardioprotective, Doxorubicin, Cichorium intybus, Nyctanthes arbor-tristis, Oxidative stress, Myocardial injury