Immunohistochemical Analysis of Cyclin D1 and Ki-67 Expression in Hyperplastic and Neoplastic Endometrial Lesions (Code-T0031)

Introduction: Endometrial hyperplasia and neoplasia are common conditions that may progress to endometrial carcinoma. Cyclin D1 and Ki-67 are cell cycle markers that regulate cellular proliferation and can be useful in distinguishing benign from malignant lesions. This study aimed to evaluate the immunohistochemical expression of Cyclin D1 and Ki-67 in hyperplastic and neoplastic endometrial lesions.

Aim and Objectives

Aim

To study Immunohistochemical expression of Cyclin D1 and Ki-67 in Hyperplastic and Neoplastic Endometrium.

Objectives

Primary Objective

1. To study the expression of Cyclin D1 and Ki-67 in Hyperplastic and Neoplastic Endometrium.

Secondary Objectives

1. To study association of Cyclin D1 and Ki-67 expression with proliferative activity of endometrium.
2. To study the association of cyclin D1 and ki-67 expression with clinicopathological features.

Methods: A total of 62 formalin-fixed, paraffin-embedded endometrial tissue samples (32 hyperplastic and 31 neoplastic) were selected. Immunohistochemical staining for Cyclin D1 and Ki-67 was performed, and the expression levels were evaluated by assessing the percentage of positive cells. The results were correlated with the histological grade of the lesions and clinical data.

Results: Cyclin D1 and Ki-67 were significantly upregulated in neoplastic lesions compared to hyperplastic lesions. High Ki-67 expression was associated with higher-grade neoplastic lesions, whereas Cyclin D1 expression showed a significant correlation with tumor differentiation. Both markers demonstrated higher proliferative activity in neoplastic lesions, with Cyclin D1 showing a stronger association with endometrial carcinoma.

Conclusion: Immunohistochemical analysis of Cyclin D1 and Ki-67 provides valuable information for differentiating between hyperplastic and neoplastic endometrial lesions. Their expression correlates with the proliferative activity and differentiation of the lesions, offering potential markers for diagnostic and prognostic purposes.

Keywords: Cyclin D1, Ki-67, endometrial hyperplasia, endometrial carcinoma, immunohistochemistry, proliferative activity.